Published in: فصلنامه آسیب شناسی ایران، دوره: 13، شماره: 4
COI code: JR_IJP-13-4_006
Paper Language: English
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Authors MiR-21 Expression in Wilms’ TumorNavid Bazzaz - Tehran university of medical sciences, Tehran, Iran
Nazila Nouraee Nouraee - Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran, Department of Experimental Therapeutics, MD Anderson Cancer Center, University of Texas, Houston, Texas,
Ali Zare-Mirzaie - Fellow of molecular pathology and cytogenetic, Tehran University Medical Sciences, Tehran, Iran. Associate professor of pathology, Iran University Medical Sciences, Tehran, Iran
Seyed Javad Mowla Mowla - Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University,Tehran, Iran
Abstract:Background and Objective: Wilms’ tumor (WT) is the most common genitourinary tract tumor in children. MicroRNAs (miRNAs) are small non-coding RNAs; their role in the pathogenesis of many types of human cancers has been identified. We aimed to evaluate the expression of miR-21, a well-known oncomir, in WT tissue samples which is a very common urinary tract malignancy in children. Methods: We performed chromogenic in situ hybridization (CISH) to detect the sub-cellular localization of miR-21 in 25 formalin-fixed, paraffin-embedded (FFPE) samples of WT. We also evaluated miR-21 expression in 24 of these blocks and 6 normal kidneys as controls using quantitative real-time PCR technique. Results: While our real-time PCR analysis showed miR-21 significant overexpression in 4 tumors compared to the normal kidney samples, we could not detect significant ISH signal in any of these samples. Conclusion: Low expression of miR-21 in WT might pinpoint the weak involvement of this miRNA in the pathogenesis of this cancer.
Keywords:Wilms’ tumor, miR-21, CISH, Real-time PCR
COI code: JR_IJP-13-4_006
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Bazzaz, Navid; Nazila Nouraee Nouraee; Ali Zare-Mirzaie & Seyed Javad Mowla Mowla, 2018, MiR-21 Expression in Wilms’ Tumor, IRANIAN JOURNAL of PATHOLOGY 13 (4), https://www.civilica.com/Paper-JR_IJP-JR_IJP-13-4_006.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Bazzaz, Navid; Nazila Nouraee Nouraee; Ali Zare-Mirzaie & Seyed Javad Mowla Mowla, 2018)
Second and more: (Bazzaz; Nouraee; Zare-Mirzaie & Mowla, 2018)
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The University/Research Center Information:
Type: Medical University
Paper No.: 6459
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