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An Efficient Method for DNA Extraction from Paraffin Wax Embedded Tissues for PCR Amplification of Human and Viral DNA

Credit to Download: 1 | Page Numbers 6 | Abstract Views: 455
Year: 2008
COI code: JR_IJP-3-4_011
Paper Language: English

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Authors An Efficient Method for DNA Extraction from Paraffin Wax Embedded Tissues for PCR Amplification of Human and Viral DNA

  Bahare Tabanifar - Dept. of Microbiology, School of Biology, Alzahra University, Tehran, Iran
  Rasoul Salehi - Dept. of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences,Isfahan, Iran
  Ezat Asgarani - Dept. of Genetics, School of Biology, Alzahra University, Tehran, Iran
  Mehri Faghihi - Dept. of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract:

Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extractionfrom formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues wereinvestigated. DNA was extracted using 45 minutes boiling in alkaline solution together with 10 beads of Chelex-20, followed by phenol-chloroform extraction and alcohol precipitation. Results: This method produced DNA suitable for amplification using primers specific for humanSMN and β globin genes in 98.63% and 82.2% of samples respectively. We also detected human papillomavirus DNA in 58.33% of appropriate samples.Conclusion: This procedure provides simple and efficient method for recovery of amplifiable genomic and viral DNA from paraffin wax embedded tissues.

Keywords:

DNA, Paraffin Embedding, Polymerase Chain Reaction

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COI code: JR_IJP-3-4_011

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Tabanifar, Bahare; Rasoul Salehi; Ezat Asgarani & Mehri Faghihi, 2008, An Efficient Method for DNA Extraction from Paraffin Wax Embedded Tissues for PCR Amplification of Human and Viral DNA, IRANIAN JOURNAL of PATHOLOGY 3 (4), https://www.civilica.com/Paper-JR_IJP-JR_IJP-3-4_011.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Tabanifar, Bahare; Rasoul Salehi; Ezat Asgarani & Mehri Faghihi, 2008)
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Type: state university
Paper No.: 5027
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