Published in: مجله طب تولید مثل ایران، دوره: 17، شماره: 8
COI code: JR_IJRM-17-8_004
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors The association between TNP2 gene polymorphisms and Iranian infertile men with varicocele: A case-control studyMohammad Mehdi Heidari - Department of Biology, Faculty of Science, Yazd University, Yazd, Iran.
Amirhossein Danafar - Department of Biology, Ashkezar Islamic Azad University, Ashkezar, Yazd, Iran.
Fahime Moezzi - Department of Biology, Faculty of Science, Yazd University, Yazd, Iran.
Mehri Khatami - Department of Biology, Faculty of Science, Yazd University, Yazd, Iran.
Ali Reza talebi - Research and Clinical Center for Infertility and Department of Anatomy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Abstract:Background: Numerous researches have provided great evidence that revealed the relationship between varicocele and sperm DNA damage. Objective: Because of the crucial role of nuclear transition proteins (TPs) in sperm DNA condensation and integrity, this case-control study was designed to study TNP2 gene nucleotide variations in Iranian patients with varicocele. Materials and Methods: PCR-SSCP and DNA sequencing were used to search for mutations in exons 1 & 2 of the TNP2 gene in 156 infertile patients with varicocele and 150 fertile men. Results: The results of sequencing showed three variants at positions c.301C > T (p.R101C), c.391C > T (p.R131 W), and g.IVS1-26G > C (rs8043625) of TNP2 gene. It was found that varicocele risk in men who have the CC genotype of g.IVS1-26G > C SNP is higher than those who don’t have these genotypes (according to Co-dominant model, Dominant model, Recessive model, and Over-dominant model). The haplotype-based analysis showed that (C/C/T) and (C/T/T) haplotypes were a risk factor of in patients with varicocele compared to controls (OR = 3.278, p = 0.000 and OR= 9.304, p = 0.038, respectively).Conclusion: Because of the significant difference in the genotype and allele frequencies of g.IVS1-26G > C SNP in the intronic region of TNP2 in patients with varicocele compared with controls and also because of the high conservation of this SNP position during evolution, this SNP may be involved in some important processes associated with the expression of this gene like mRNA splicing, but the exact mechanism is not clear.
Keywords:Varicocele, Genetic polymorphism, Infertility, Nuclear transition protein 2.
COI code: JR_IJRM-17-8_004
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Heidari, Mohammad Mehdi; Amirhossein Danafar; Fahime Moezzi; Mehri Khatami & Ali Reza talebi, 2019, The association between TNP2 gene polymorphisms and Iranian infertile men with varicocele: A case-control study, International Journal of Reproductive BioMedicine 17 (8), https://www.civilica.com/Paper-JR_IJRM-JR_IJRM-17-8_004.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Heidari, Mohammad Mehdi; Amirhossein Danafar; Fahime Moezzi; Mehri Khatami & Ali Reza talebi, 2019)
Second and more: (Heidari; Danafar; Moezzi; Khatami & talebi, 2019)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
The University/Research Center Information:
Type: state university
Paper No.: 11274
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- Clomiphene citrate impairs the endometrial CD98 expression in ovariectomized and non-ovariectomized rats: Role of HCG
- Diabetes mellitus increased integrins gene expression in rat endometrium at the time of embryo implantation
- Expression of miR-302 in human embryo derived from in-vitro matured oocyte
- Aphrodisiac property of aqueous and methanolic extracts of Raphia vinifera (Arecaceae) in sexually experienced male rats
- Beneficial effects of Cichorium intybus L. extract on oxidative status and reproductive parameters in male Wistar rats: An experimental study
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.