Downregulation of Drosha, Dicer, and DGCR8 mRNAs in Peripheral Blood Mononuclear Cells of Patients with Rheumatoid Arthritis

Publish Year: 1397
نوع سند: مقاله ژورنالی
زبان: English
View: 351

This Paper With 9 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_RHRE-3-4_003

تاریخ نمایه سازی: 1 خرداد 1398

Abstract:

Rheumatoid arthritis (RA) is a systemic autoimmune disorder causing irreversible joint damage. MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that degrade or translate inhibition of mRNAs. miRNAs can be used as therapeutic targets and predictive biomarkers in many disordres. This study was undertaken to investigate whether or not the expression of key elements in miRNA biogenesis, Drosha, DGCR8 and Dicer mRNAs is dysregulated in RA patients.In this case-control study, 50 patients with RA and 50 age- and gender-matched healthy subjects participated. The peripheral blood mononuclear cells (PBMCs) were separated from the whole blood, the total RNA content of the cells was isolated and the first strand cDNA was synthesized. Quantitative analysis was performed through real-time polymerase chain reaction (PCR) using SYBR Green gene expression master mix to detect mRNA level expression of Drosha, DGCR8 and Dicer.The expression levels of Drosha and DGCR8 were significantly downregulated in patients with RA in comparison with the healthy controls (P value = 0.043, P value = 0.000365, respectively). The expression level of Dicer was downregulated in RA patients when compared to the healthy controls, although the difference in expression was not significant (P value= 0.156). RA patients with a familial history of autoimmune rheumatic disease recorded significant overexpression of all three genes. Moreover, DAS28 was significantly correlated with mRNA exoressiom of Drosha, Dicer and DGCR8.The data suggests that downregulated expression of Drosha, DGCR8 and Dicer mRNAs may be contributing to the pathogenesis of RA.

Authors

Maassoumeh Akhlaghi

Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran

Samaneh Soltani

Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran

Fatemeh Jamshidi

Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran

Seyedeh Tahereh Faezi

Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • Asadipour M, Hassan‐Zadeh V, Aryaeian N, Shahram F, Mahmoudi M. ...
  • Scott DL. Biologics-based therapy for the treatment of rheumatoid arthritis. ...
  • Xu WD, Zhang M, Zhang YJ, Ye DQ. IL-33 in ...
  • Suzuki A, Yamada R, Chang X, Tokuhiro S, Sawada T, ...
  • Brooks WH, Le Dantec C, Pers JO, Youinou P, Renaudineau ...
  • Pillai RS. MicroRNA function: multiple mechanisms for a tiny RNA ...
  • Zisoulis DG, Kai ZS, Chang RK, Pasquinelli AE. Autoregulation of ...
  • Chen CZ, Li L, Lodish HF, Bartel DP. MicroRNAs modulate ...
  • Xiao C, Rajewsky K. MicroRNA control in the immune system: ...
  • Dooley J, Linterman MA, Liston A. MicroRNA regulation of T‐cell ...
  • Hussain N, Zhu W, Jiang C, Xu J, Wu X, ...
  • Furer V, Greenberg JD, Attur M, Abramson SB, Pillinger MH. ...
  • Bogunia-Kubik K, Wysoczanska B, Piatek D, Iwaszko M, Ciechomska M, ...
  • Balzano F, Deiana M, Dei Giudici S, Oggiano A, Pasella ...
  • Song GG, Bae SC, Seo YH, Kim JH, Choi SJ, ...
  • Chen ZZ, Zhang XD, Chen Y, Wu YB. The role ...
  • Sand M, Gambichler T, Skrygan M, Sand D, Scola N, ...
  • Gregory RI, Chendrimada TP, Cooch N, Shiekhattar R. Human RISC ...
  • gene silencing. Cell 2005; 123(4):631-40.doi:10.1016/j.cell.2005.10.022. ...
  • Han J, Pedersen JS, Kwon SC, Belair CD, Kim YK, ...
  • Han J, Lee Y, Yeom KH, Kim YK, Jin H, ...
  • Rostami Mogaddam M, Safavi Ardabili N, Shafaeei Y, Maleki N, ...
  • Dalmay T. MicroRNAs and cancer. J Intern Med 2008;263(4):366-75.doi:10.1111/j.1365- 2796.2008.01926.x. ...
  • Tavasolian F, Abdollahi E, Rezaei R, Momtazi-Borojeni AA, Henrotin Y, ...
  • Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, ...
  • 7 revised criteria for the classification of rheumatoid arthritis. Arthritis ...
  • Sand M, Skrygan M, Georgas D, Arenz C, Gambichler T, ...
  • Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, ...
  • Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the ...
  • Asadipour M, Hassan-Zadeh V, Aryaeian N, Shahram F, Mahmoudi M. ...
  • Zhu J, Huang X, Su G, Wang L, Wu F, ...
  • Otaegui D, Baranzini SE, Armananzas R, Calvo B, Munoz-Culla M, ...
  • Lovendorf MB, Mitsui H, Zibert JR, Ropke MA, Hafner M, ...
  • Zilahi E, Tarr T, Papp G, Griger Z, Sipka S, ...
  • Mookherjee N, El-Gabalawy HS. High degree of correlation between whole ...
  • Tang X, Yin K, Zhu H, Tian J, Shen D, ...
  • Filkova M, Aradi B, Senolt L, Ospelt C, Vettori S, ...
  • Murata K, Yoshitomi H, Tanida S, Ishikawa M, Nishitani K, ...
  • Ogando J, Tardaguila M, Diaz-Alderete A, Usategui A, Miranda-Ramos V, ...
  • Pauley KM, Satoh M, Chan AL, Bubb MR, Reeves WH, ...
  • Papachristou DJ, Korpetinou A, Giannopoulou E, Antonacopoulou AG, Papadaki H, ...
  • Chong MM, Rasmussen JP, Rudensky AY, Littman DR. The RNAseIII ...
  • Saeki M, Watanabe M, Inoue N, Tokiyoshi E, Takuse Y, ...
  • Rostami Mogaddam M, Safavi Ardabili N, Shafaeei Y, Maleki N, ...
  • Jafari N, Shaghaghi H, Mahmoodi D, Shirzad Z, Alibeiki F, ...
  • Tabrizi Z, Mansouri R, Aslani S, Jamshidi A, Mahmoudi M. ...
  • Wang S, Yuan M, Song L, Zhang X, Geng Q, ...
  • Castro-Villegas C, Pérez-Sánchez C, Escudero A, Filipescu I, Verdu M, ...
  • نمایش کامل مراجع