Evaluation of antibiotic resistance pattern among group B Streptococci isolated from urinary tract infection of teaching hospitals in Isfahan.

Background: Group B Streptococci (GBS) is an important bacterial pathogen that causes a wide range of infections including neonatal sepsis, meningitis, pneumonia, soft tissue and urinary tract infections(UTI). The aim of this study was to evaluate the antibiotic susceptibility patterns of GBS isolates obtained from patients with UTIs referred to teaching hospitals of Isfahan, Iran. Methods: In this cross-sectional study, Streptococcus agalactiaeisolates were collected from urine specimens of patients with UTIs referred to teaching hospitals of Isfahan, Iran. Bacterial isolates were identified using standard microbiological methods; including Gram staining, catalase, oxidase, CAMP, and genotypic method (the presence of the dlts gene). Antibiotic susceptibility was performed based on disk diffusion method on Mueller–Hinton agar with sheep blood (5% v/v) and alsoPenicillin-nonsusceptible isolates were teste with the E-test. according to the Clinical and Laboratory Standards Institute (CLSI).S.aureus ATCC 25923 was also used as the positivecontrol. Results: One hundred of UTI isolates confirmed as GBS. Antibacterial susceptibility pattern showed that 95% of GBS isolates was resistant totetracycline, followed by erythromycin (52%), clindamycin(47%), levofloxacin(9%) andcefepime, cefotaxime,and ceftriaxone each with (8%), while, vancomycin was the most effective antibiotic (99%). Moreover, eightisolate showed reduced susceptibility pattern to penicillin (nonsusceptible, MICs > 0.12). Conclusion: In summary, our results showed reduced sensitivity to antibiotics, especially against penicillinin S.agalactiae isolates. According to theimportance of GBS in children, pregnant women, and elderly people with chronic underlying diseases andpenicillinwhichis accounted thedrug of choice for GBS eradication, attention to the antibiotic susceptibility pattern of S.agalactiae cause UTI is necessary.

Keywords:

Antibiotic resistance , Group B Streptococci , Urinary tract infection