IN SILICO IDENTIFICATION AND COMPARATIVE GENOMICS OF CANDIDATE GENES INVOLVED IN BIOSYNTHESIS NATURAL PRODUCTS IN CYANOBACTERIA STRAINS OF IRAN
Publish place: 19th International Congress of Microbiology of Iran
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
MEDISM19_111
تاریخ نمایه سازی: 13 مهر 1397
Abstract:
Background and Aim:Cyanobacteria have the ability to produce a wide variety of natural bioactive compounds. Horizontal gene transfer is thought to play a role in the sporadic distribution of bioactive compounds producers among cyanobacteria. The most prevalent cyanobacterial natural products are produced in combinatorial pathways like mixed PKS/NRPS systems. Nonribosomal peptide synthetases (NRPSs) and polyketide synthase (PKSs), synthesize a diverse array of bioactive small peptides, many of which are used in medicine. Methylprolines are non proteinogenic amino acids produced by cyanobacteria. Methods:To investigation the relationship between natural product genes and the role of horizontal gene transfer in the evolutionary history, we amplified and sequenced the NRPS, PKS and Methylprolines biosynthetic genes from from 25 cyanobacteria strains isolated from different Climatic/Geographic Regions and Habitats. The analyses were done using a combination of the genome mining softwares, AntiSMASH, and NaPDoS. Moreover, adenylation domain substrate specificity predictions for NRPS enzymes, the signature sequences and the name of the compounds, were made using NRPSpreditor2. In addition; we detected Methylprolines biosynthetic gene from 8 strains included in this study. Results:Our results suggest that Methylprolines biosynthetic genes have a complex evolutionary history in cyanobacteria, which has been punctuated by a series of ancient horizontal gene transfer events and probably the ability of lateral gene transfer to produce the natural bioactive compound has been lost during evolutionary history in these strains.Conclusion:The data presented here highlight the need for future studies to define the linkages between horizontal gene transfers in maintenance of bioactive compounds genes.
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Authors
Bahareh Nowruzi
Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. ORCID: ۰۰۰۰-۰۰۰۱-۶۶۵۶-۷۷۷X
Fatemeh Zahedi
Department of Microbiology, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University ,Tehran - Iran (IAUPS )