ANTIBACTERIAL EFFECT OF AILANTHUS ALTISSIMA EXTRACT ON SOME BACTERIA IN SWIMMING POOLS IN COMPARISON WITH ANTIBIOTICAL EFFECT
Publish place: 19th International Congress of Microbiology of Iran
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
MEDISM19_267
تاریخ نمایه سازی: 13 مهر 1397
Abstract:
Background and Aim:Swimming pools are infected with bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris that enter the pool through body open pores due to inaccuracy and non-compliance with sanitary regulations.Methods:In this research, a fast-growing plant of Ailanthus altissima was used that is now considered as an ecological problem. The leaves were collected and powdered in the shade. The aqueous, hydroalcoholic and methanolic extracts were prepared by maceration and condensed by rotary. Four concentrations of 20, 30, 40 and 50 mg/ml of extracts were used. For the effectiveness of extracts, the agar well method and nutrient agar medium were used and each effect was replicated three times. Chloramphenicol antibiotic was used as a positive control.Results:Halo diameter of lack of microbial growth was proportional to the concentration of extract. The results showed that methanolic A. altissima extract with a concentration of at least 30 mg/ml had the highest halo of lack of microbial growth for all three bacteria. The most effectiveness of methanolic extract of A. altissima was on P. vulgaris (with halo diameter of lack of microbial growth of 19-20 mm).Conclusion:Although the lower concentration of antibiotics has the same result, the use of a plant extract with a higher concentration, will be uncomplicated, or of at least minimal complication.
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Authors
Razieh Taghavizad
Associate professor of the Department of Biology, Yadegar -e- Imam Khomeini (RAH) Shahre-Rey Branch, Islamic Azad University, Tehran, Iran
Sepideh Farazandeh
M.Sc. Student of Microbiology, Yadegar -e- Imam Khomeini (RAH) Shahre-Rey Branch, Islamic Azad University, Tehran, Iran.
Shiva Khalil-Moghaddam
Associate professor of the Department of Biology, Yadegar -e- Imam Khomeini (RAH) Shahre-Rey Branch, Islamic Azad University, Tehran, Iran