BACTERIAL MAGNETIC NANOPARTICLES CONJUGATED WITH MONOCLONAL ANTIBODY AS AFFINITY-MAGNETIC MATRIX AND APPLICATION IN AFFINITY MAGNETIC SEPARATION

Background and Aim:Biogenic magnetic nanoparticles (MNPs) synthesized by magnetotactic bacteria due to their individual features and have great potential in biomedical applications recently fascinated great attention. Bacterial magnetosomes have been used experimentally as the carriers for enzymes, nucleic acids, antibodies and anticancer drugs since 1991. In addition to the unique applying properties of biogenic magnetic carriers, magnetosomes also show superiority as multifunctional targeting nanoscale drug carriers, which is hardly matched by synthetic magnetic particles. Conjugation of monoclonal antibodies to magnetosome nanoparticles is an effective method for macromolecules diagnosis and purification.Methods:. In this study, the monoclonal antibodies against 24 kDa surface antigen of hepatitis B viruses were conjugated to super biogenic paramagnetic iron oxide (magnetosome) nanoparticles using Sulfo-LC-SPDP and Sulfo SMCC method in order to separate the HBsAg.Results:The magnetic separation of this conjugated monoclonal antibody was found to be 28% higher than affinity chromatography method that can be used to the best alternative method instead of it s for the isolation and purification of recombinant HBsAg from yeast for vaccination. Also, studies with a Zeta Potential Analyzer showed that the magnetosomes-Ab complex had hydrated radii significantly smaller than those of WT magnetosomes and zeta potentials less than −40 mV, indicating that the magnetosome-Ab colloids were relatively stable.Conclusion:Observed magnetosome-Ab conjugation efficiencies were as high as 71.24 μg Ab per mg magnetosomes nanoparticles, and the conjugated Abs retained most of their activity.

Keywords:

magnetosome , Magnetospirillum gryphiswaldense MSR-1 , affinity magnetic separation , cojugation. monoclonal antibody