INCREMENT OF LYSOBACTER ENZYMOGENES PROTEASE ACTIVITY BY EMPLOYING COLD ATMOSPHERIC PLASMA

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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MEDISM19_352

تاریخ نمایه سازی: 13 مهر 1397

Abstract:

Background and Aim:Cold atmospheric plasma (CAP) is an ionized gas that has recently been extensively studied. It has mutagenic effect which can be used as a method of random mutagenesis in order to obtain improved strains at the industrial scale. In the present study, the effect of CAP was assessed for the first time on the growth and physiological characteristics of Lysobacter Enzymogenes which is the bacteria known for producing endopeptidases. Moreover, screening was done to define isolates with higher production rate of protease enzyme.Methods:The L. Enzymogenes , ATCC 29487 was cultured in the nutrient agar medium for 48 hours at 33° C. Duration of exposure to CAP was increased gradually for 20, 45, 60, 90, 120, 150, 180 seconds in a total volume of 400 microliters of the overnight culture of the bacteria. Then the dilution of mutated bacteria was cultured in nutrient agar medium to obtain single colonies. Screening was performed using casein agar and by measuring the diameter of the halo zone in the skimmed milk agar. Then the protease activity of selected strains was determined by Lowry method.Results:The results showed that CAP was not hampering the growth of the bacteria even at the exposure of 180 second. After screening, some mutated strains showed a larger halo zone diameter and increased enzymatic activity compared to the wild-typed bacteria.Conclusion:CAP didn’t showed destructive effect on the bacteria growth and acted as a physical mutagen for obtaining enhanced L. Enzymogenes strains which showed higher protease production rate.

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Authors

Faranak Faraji Tabar

Islamic Azad University, Science and Research Branch, Tehran

Vahideh Valizadeh

Pasteur Instite Of Iran, Nanobiotechnology department

Seyed Mohammad Atyabi

Pasteur Instite Of Iran, Nanobiotechnology department

Malihe Keramati

Pasteur Instite Of Iran, Nanobiotechnology department