ANTIBACTERIAL EFFECTS OF CEFTIZOXIME COMBINED WITH METAL NANOPARTICLES AGAINST NEISSERIA GONORRHOEAE

Background and Aim:Neisseria gonorrhoeae is the second most prevalent bacterial sexually transmitted infection (STI) worldwide, and the etiological agent, N. gonorrhoeae has developed resistance to all antimicrobials used as first-line treatments. The aim of this study was to formulate CuO and Ag nanoparticles Coated by Ceftizoxime and determine its antibacterial effects in comparison with common antibiotics against N. gonorrhoeae.Methods:N. gonorrhoeae PTCC 1773 was obtained from the Iranian Research Organization for Science and Technology (IROST). The nanoparticles were prepared using chemical reduction method. The morphology of the nanoparticles was observed using an XRD and SEM. Antimicrobial effect of Nano formulations were investigated using well diffusion method. Minimum Inhibitory Concentrations (MIC) were determined by monitoring the growth of bacteria at 600 nm after 24 hours incubation at 35°C.Results:In this study, the antimicrobial activity of nanoforumulation for N. gonorrhoeae was measured by well diffusion method. Antibiotic conjugation with nanoparticles shows a higher antibacterial function than any of the formulations alone.Conclusion:The antibacterial activity of Ceftizoxime was enhanced in presence of metal nanoparticles against N. gonorrhoeae. This allows more efficient therapy compared with the antibiotic original form. Ceftizoxime@metal nanoparticles could be used as an adjuvant with antibiotic therapy, either for topical use or as a coating for biomaterials, to treat N. gonorrhoeae.

Keywords:

Metal Nanoparticles , Ceftizoxime , Neisseria gonorrhoeae