APPLICATION OF OPTAMER IN THE DETECTION OF AFLATOXIN B1

Background and Aim:: In order to detect aflatoxin B1, a fluorescence assay has been developed using the fluorophore labeled optamer and its partial complement (a single-stranded DNA that has been modified as a covalently by the IOWA BLACK T quencher) in this studyMethods:The overall measurement method is simple and fast and has a high sensitivity to aflatoxin B1, which has a great potential to on-site analysis. In the absence of aflatoxin B1, optamamer binds to the cDNA, and fluorophore absorbs the quencher and neutralizes the fluorescence effect. In the presence of Aflatoxin B1, the optamer forms an optamer / aflatoxin B1 complex and results in the release of cDNA that is associated with fluorescence. The fluorescence of optamer, optamer with cDNA and optamer with cDNA and aflatoxin were measured using Trilog f7000 fluorometer.Results:Using the design expert software, the optimal concentrations of optamer and cDNA were determined to be 10 and 15 nM, respectively. Fluorescence recovery curve versus aflatoxin B1 concentration was linear (r2 = 0.9942). The detection limit of this method with a standard curve of 10-100ppb is 8 / 47ppb. This method was used for potato starch.Conclusion:This optasensor is suitable to detect aflatoxin B1in foods.

Keywords:

optamer , quenching , potato starch , aflatoxin