APPLICATION OF MINITAB SOFTWARE ON THE OPTIMIZATION OF INACTIVATION CONDITIONS OF RABIES VIRUS USED IN VET RABIES VACCINE BY BETA-PROPIOLACTONE

Background and Aim:Veterinary rabies vaccine which is routinely produced contains tissue culture-adapted rabies Pasteur Virus strain propagated on BHK-21 cells. Various methods can be used for inactivating the rabies virus, such as treatment with β propiolactone , acetylethyenemine or ultraviolet irradiation.Methods:In present study, Pasteur Virus strain grown was treated with BPL based on Mini Tab program by design of excremental and used Two Level Factorial Design and Central Composite Design method in 3 factors and 3 levels which in final design twenty base runs, sixty totals run with final blocks: 1 carried out. Each sample titrated after various intervals and tested for the viral infectivity in BSR cells and in vivo inactivity test carried out on mice.Results:Based on in vivo and in vitro obtained results, absolute loss of infectivity of the virus treated with 1:3000, 1:4000 and1:5000 final concentrations of BPL were evident at the end of the 72 h in 21◦C and 4◦C.In each samples at the end of the 72 hr and 120 hr in 4°C and 21°C did not any infectivity of the virus. Complete loss of infectivity of the virus treated with 1:3000, 1:4000 of BPL was evident at the end of the 48 hours hr in 4°C and 21°C.Conclusion: The aim of this study was to evaluate the effects of temperature, time of exposure and concentrations of BPL on the infectivity of the rabies virus, in order to achieve the ideal conditions for inactivation of the virus, harvested from the cell cultures.

Keywords:

Optimization , BPL , Variables