COMPARED PROTEIN PROFILES OF THE OUTER MEMBRANE VESICLES (OMV) AND OUTER MEMBRANE PROTEINS (OMP) STANDARD STRAINS OF AKKERMANSIA MUCINIPHILA

Background and Aim:The gastrointestinal tract containing 10 to 100 trillion bacteria and other microorganisms. Gastrointestinal microbiota play an important role in human health, the permeability of the intestinal barrier, the distribution of fat mass, and energy homeostasis. Change in patterns of gut microbiota, increases permeability of the intestinal barrier, and increases the plasma LPS that leads to metabolic syndrome disease including obesity. A.muciniphila is a gram-negative anaerobic bacteria and gang of from 3-5% of intestinal microbial community. This bacteria is considered as a positive health index of individuals The aim of this study, was compared protein profiles of the outer membrane vesicles (omv) and outer membrane proteins (omp) standard strains of Akkermansia muciniphilaMethods:In this method first of all we cultured lyophilize of standard strains of Akkermansia muciniphila then after enrichment we can extract Omv and Omp ; To study physicochemical properties, scanning electron microscope ,nano drop , LAL, pyrogenic test were done . Finally we assayed proteins with SDS page methodResults:it is observed that the sampels non pyrogenic,the range size of omv in SEM is from 36 to 153 nm , the band range of omv is from 245 to 75 kd and omp is from 180 to 100 kdConclusion:Finally because of significance of A.muciniphila as a remarkeabl common member of the human gut microbiota and its beneficial influence in curing diseases, the diagnosis of protein profile A.muciniphila can be help full in Immunology and Molecular studies.

Keywords:

Akkermansia muciniphila , omv , omp