ALTERATION IN GST ACTIVITY IN LIVER, KIDNEY AND SPLEEN TISSUE OF IMMUNIZATION RAINBOW TROUT (ONCORHYNCHUS MYKISS) AGAINST ICHTHYOPHTHIRIUS MULTIFILIIS

Background and Aim:Ichthyophthirius multifiliis is a large ciliated protozoan parasite with distribution of throughout the world. Vaccination against I. multifiliis can be considered as the option ways to chemotherapy in fish. Because of not handling fish stocks, non-stressful method and less time consuming; oral administration can be considered as a good candidate. To improve the effectiveness of oral vaccination, development of an efficient delivery method is of major importance. The aim of present study was to clarify the effects of nanoparticles system for protection of the irradiated trophonts on glutathione S-transferase (GST) as oxidative stress biomarker in liver, kidney and spleen tissues of immunized rainbow trout against I. multifiliis.Methods:A total of 150 parasite free rainbow trout were randomly assigned to 5 groups in triplicate, 30 fish per each aquarium. Group 1 served as healthy control. Group 2 was administered with gamma-irradiation trophonts. A group 3 was administered with alginate nanoparticles. A group 4 was administered with encapsulated gamma-irradiatied trophonts with alginate nanoparticles. A group 5 was non-immunized but infected with 10000 live trophonts. After sampling, GST activity in the tissues was assayed by using commercially available kits.Results:GST activity in the liver, kidney and spleen tissues of rainbow trout treated with encapsulated gamma-irradiatied trophonts with alginate nanoparticles was significantly higher than the other groups.Conclusion:Therefore, these results suggest that alginate nanoparticles could be more useful for development of gamma irradiated trophonts as radiovaccine

Keywords:

glutathione S-transferase (GST) , Alginate nanoparticles , Gamma irradiated I. multifiliis trophonts