ANTIMICROBIAL ACTIVITY AND ANTIBIOTIC SUSCEPTIBILITY OF BIFIDOBACTERIUM SPP ISOLATE FROM THE FECES OF HEALTHY INFANT AGAINST HOSPITAL , ENTEROPATHOGENIC AND FOOD-BORNE PATHOGENS

Background and Aim:The human intestinal microflora is complex with total counts of 1011-1012 bacteria per gram of stool.The research comprises isolation and identification of Bifidobacterium strains from the feces of healthy infant.Methods:samples cultured in MRS broth, Bifido agar under anaerobic conditions.Identification of Bifidobacterium isolates was performed by biochemical test and PCR technique using species specific Primers . An agar well diffusion assay , disc diffusion method and Elisa method was used for detection of antimicrobial activity,Antibiotic susceptibility and Aflatoxin B1 detoxification potential respectively.Statistical analyses were performed with SPSS software.One-way ANOVA with post-hoc Tukey HSD was used for statistical analysis. Results were regarded as statistically significant at p< 0.05Results:The examined strains were identified as B. Longum , B. bifidum and B. Breve .All Isolate showed good probiotic potential.The majority of the strains exhibited antagonistic activity towards Bacillus Cereus , salmonella , Campylobacter , E.coli , shigella , staphylococcus areus respectively . all strains tested were susceptible to penicillin G, amoxicillin, piperacillin, ticarcillin, imipenem, chloramphenicol, rifampicin and vancomycin . all strains tested were resistant to gentamicin, sulfamethoxazole and polymyxin B . Most isolates were resistant to fusidic acid .Conclusion:This study showed that Bifidobacterium strains with good probiotic potential could be isolated from fecal of healthy infant and suggest Bifidobacterium strains with probiotic potential may be useful for prevention or treatment of diarrhea and Supportive therapy in Aflatoxicosis but further in vitro and in vivo studies on these strains are still required.

Keywords:

Bifidobacterium , Infant , Fecal flora , Probiotics , Antimicrobial Activity