Frequency of Gardnerella vaginalis in patients with vaginosis in Isfahan by molecular method

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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MEDISM20_019

تاریخ نمایه سازی: 26 بهمن 1398

Abstract:

Introduction and objectives: Bacterial vaginosis is a common vaginal disorder characterized by a change in bacterial vaginal microbial complexity from a normal to an inhomogeneous state containing a complex population of organism mainly anaerobic and micro aerobic. Gardnerella vaginalis is a gram variable coccobacilli and non-moving that can grow in micro aerobic. Selective treatment for Gardnerella vaginosis is metronidazole and clindamycin, both of which are available in the form of oral capsules and vaginal ointment, but antibiotic resistance has been reported in recent years. Materials and Methods: In this cross-sectional study, 110 women referring to Isfahan clinics for bacterial vaginosis were studied. Patient satisfaction was obtained and a patient-specific questionnaire was prepared, diagnosis of the disease with 4 pH-based laboratory methods, whiff test, observation of clue cell in direct incubation and secretion culture in specialty chocolate agar containing starch and extracts of heart and brain containing starch. Confirmation of identification was carried out by biochemical tests. Also, all samples were evaluated for molecular detection of Gardnerella vaginalis.Results: Based on molecular identification from a total of 110 patients with bacterial vaginosis symptoms, 32 (29.09%) had bactericidal vaginosis due to Gardnerella vaginalis. The characteristics of patients with bacterial vaginosis and non-infected individuals were compared. All women with this infection were married and most of them (43.8%) belonged to the age group of 25-30 years old. Among the patients (59.37%), infected were resident in the village and non-infected (54.54%) in the city. There was no significant difference between the two groups in terms of their place of residence. There was no significant difference in terms of education, occupation, number of previous pregnancies, abortion rates, use of antibiotics, vaginal ointment, and rate of secretion between the two groups. The results of chi-square test showed a significant difference between patients with positive clue cell in both groups of infected and non-infected to Gardnerella vaginalis (P <0.01). Conclusion: The relatively high frequency of Gardnerella vaginalis in women with bacterial vaginosis indicates the importance of the role of bacteria in the incidence of bacterial vaginosis. Also, in the studied region, bacterial vaginosis is prevalent and has many complications and it is necessary that people at risk be screened and if necessary be treated.

Authors

N Mohammadi

Department of Microbiology, Falavarjan Branch, Islamic Azad University, Esfahan, Iran

M Mohammadi-sichani

Department of Nursing and Midwifery, Falavarjan Branch, Islamic Azad university, Esfahan, Iran

M Allahdadian

Department of Microbiology, Falavarjan Branch, Islamic Azad University, Esfahan, Iran