Survey major toxins of Clostridium perfringens isolated of ruminants by molecular method in the southeast of Iran

Introduction and Objectives: Clostridium is a common bacterium that is responsible for involving live stocks; poultries even humans and may lead to large economic losses. Clostridium perfringens (C. perfringens) produces a lot of toxins. This is divided into five types, from A to E, based on their ability to produce any of the four major lethal toxins (alpha, beta, epsilon and iota). The aim of this research is identifying of various genotypes of C. perfringens in the southeast of Iran. Materials and Methods: Sampling strategies was according to the livestock population. Firstly samples were cultured on enriched medias under anaerobic conditions after that some differential medias used for culture and identification of C. perfringens speies . DNA extraction of all isolated carried out and multiplex PCR reaction was performed by using specific primers to indicate types of C. perfringens. Results: The results showed that upon of half of isolated was type A in three groups (sheep, goat and cow), furthermore The type B was less than other types. Phylogenic tree, nucleic acids affinity, comparison of genetic similarity percent of C. perfringens isolates with standard strains and submitted gene in Gen Bank amplified that the same reaction fragments by PCR related to C. perfringens. Conclusion: C. perfringens type A was dominant than other types. These isolated strains can be used in research and manufacturing projects.

Keywords:

Clostridium perfringens , Diagnosis , Toxin , Multiplex PCR.