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title

Effect of lactobacillus plantarum consumption on hematologic parameters in rats with bone marrow inflammation

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Year: 2019
COI code: MEDISM20_058
Paper Language: English

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Authors Effect of lactobacillus plantarum consumption on hematologic parameters in rats with bone marrow inflammation

  Sajedeh Mahpishanian - Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
  Fereshteh Ghandehari - Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
  Mehrnosh Fatemi - Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

Abstract:

Introduction and Objectives: Bone marrow inflammation is a disease that affects the entire bone. The most important pathogen responsible for this disease is Staphylococcus aureus which penetrates the osteoblasts from the immune system and therefore does not respond to common antibiotic treatments. It has been recently shown that Probiotics enriched with selenium have a high inhibitory effect on pathogens. in the present study Effect of lactobacillus plantarum consumption on hematologic parameters in rats with bone marrow inflammation Was investigated After induction Bone inflammation with Staphylococcus aureus. Materials and Methods: in this study the number of 26 male wistar rats was prepared and divided into 6 separate groups including: control group(infected with staphylococcus areus), group infected with staphylococcus areus that treated with lactobacillus plantarum, group inoculated with staphylococcus areus that treated with selenium enriched lactobacillus plantarum, Piercing the foot bone and injecting 100 μl of Staphylococcus aureus suspension has done in group that was inoculated with staphylococcus areus. after treatment, blood sampling was directly performed from the animal heart. The number of blood cells and their indexes were analyzed using MINDRAY CBC device. After the completion of blood sampling, the bone marrow of the right leg of the rats was carefully separated from the middle of the bone and then homogenized in Serum Physiology buffer, and then the supernatant isolated after centrifugation. 100 μl of supernatant was cultured in Muller hinton Agar medium and incubated at 37°C for 24hrs. the number of bacteria was counted after incubation.Results: Results showed that the level of White blood cells in group inoculated with staphylococcus areus(P≤0/001) and group inoculated with staphylococcus areus that treated with lactobacillus plantarum(P≤0/05) increased. Although the level of them were increased in group infected with staphylococcus areus that treated with selenium enriched lactobacillus plantarum, but there was no significant difference with control group. The variations in other factors in the experimental groups were not significant in comparison to the control group. Number of single Staphylococcus aureus colonies in group inoculated with staphylococcus areus and in group that treated with selenium enriched lactobacillus plantarum, was significantly decreased in compared to group inoculated with staphylococcus areus .while the number of this colonies in group treated with lactobacillus plantarum showed no variation in compared to group infected with staphylococcus areus. Conclusion: It seams that selenium enriched lactobacillus plantarum reduced infections caused by staphylococcus areus through it’s antioxidant and antibacterial activity and thos leads to reduction of white blood cells and number of staphylococcus areus colonies. While other blood parameters are not affected by this condition.

Keywords:

Acute bone marrow inflammation, Staphylococcus areus, lactobacillus plantarum, Selenium

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COI code: MEDISM20_058

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Mahpishanian, Sajedeh; Fereshteh Ghandehari & Mehrnosh Fatemi, 2019, Effect of lactobacillus plantarum consumption on hematologic parameters in rats with bone marrow inflammation, 20th International Congress of Microbiology of Iran, كرمان, دانشگاه علوم پزشكي كرمان, https://www.civilica.com/Paper-MEDISM20-MEDISM20_058.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Mahpishanian, Sajedeh; Fereshteh Ghandehari & Mehrnosh Fatemi, 2019)
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Type: Azad University
Paper No.: 397
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