Published in: 20th International Congress of Microbiology of Iran
COI code: MEDISM20_091
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors An Investigation into Antibacterial Activity of Fluoroquinolone-Derived Compounds on Two Gram-Negative Bacteria: Escherichia coli and Pseudomonas aeruginosaAssar Shokrollah - Rafsanjan University of Medical Sciences
Ali Darehkordi - Valiasr University, Rafsanjan, Kerman
Gholamhossein Hassanshahi - Rafsanjan University of Medical Sciences
Abstract:Introduction and objectives: Quinolones are known as a bunch of antibiotics inhibiting two central enzymes involved in DNA replication and transcription; i.e. DNA gyrase and topoisomerase IV. Among them, fluoroquinolones can be developed via substituting fluorine atoms at the sixth position of core quinolone structure, thereby enhancing antibacterial activity. As a result, growth and proliferation of bacteria may be prevented through new compounds derived from fluoroquinolones and somehow strengthen their antibacterial effects. Materials and Methods: The present study was designed and conducted to measure inhibitory concentrations of N-4-methyl (phenyl)-2,2,2-trifluoroacetimidoylciprofloxacin (5a) and N-4-methyl (phenyl)-2,2,2- trifluoroacetimidoylnorfloxacin (5b) as two synthetic derivatives of fluoroquinolones. In addition, real-time PCR (RT-PCR) technique was used to assay the performance of these two derivatives on DNA gyrase gene expression levels in Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) as gram-negative bacteria. Broth microdilution method and disc diffusion test were also employed to determine minimum inhibitory concentration (MIC) of these synthetic compounds in comparison with conventional antibiotics of gentamicin and ciprofloxacin (a fluoroquinolone). Results: According to the findings; 5a compared with the two antibiotics in gram-negative bacteria showed inadequate efficacy at phenotypic and molecular levels, and it could also have antibacterial effects assumed less than the given antibiotics. In contrast, 5b generated a larger diameter of the zone of inhibition (ZOI) in E. coli and P. aeruginosa compared with the two antibiotics that were reported statistically significant (p=0.00). The results of the broth microdilution method also confirmed findings from disc diffusion test. On the other hand, 5b brought about a significant reduction of DNA gyrase expression levels in both bacteria, while 5a had did not show such a significant effect in this domain. Conclusion: The results of this study suggested that 5b could be used as a new and alternative antibiotic candidate for gentamicin or ciprofloxacin against infections caused by E. coli and P. aeruginosa. However, further research focused on various dimensions, including corresponding complications, as well as clinical trials were required to draw a definite conclusion on these synthetic compounds.
Keywords:Fluoroquinolone, N-4-Methyl (Phenyl)-2,2,2-Trifluoroacetimidoyl Ciprofloxacin (5a), N-4-Methyl (Phenyl)-2,2,2-Trifluoroacetimidoyl Norfloxacin (5b), Escherichia coli, Pseudomonas aeruginosa
COI code: MEDISM20_091
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Shokrollah, Assar; Ali Darehkordi & Gholamhossein Hassanshahi, 2019, An Investigation into Antibacterial Activity of Fluoroquinolone-Derived Compounds on Two Gram-Negative Bacteria: Escherichia coli and Pseudomonas aeruginosa, 20th International Congress of Microbiology of Iran, كرمان, دانشگاه علوم پزشكي كرمان, https://www.civilica.com/Paper-MEDISM20-MEDISM20_091.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Shokrollah, Assar; Ali Darehkordi & Gholamhossein Hassanshahi, 2019)
Second and more: (Shokrollah; Darehkordi & Hassanshahi, 2019)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
The University/Research Center Information:
Type: Medical University
Paper No.: 288
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.
Research Info Management
Export Citation info of this paper to research management softwares
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.