Detection of Coxiella burnetii in bulk tank milk in dairy cattle farm of Kerman by nested-trans PCR

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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MEDISM20_201

تاریخ نمایه سازی: 26 بهمن 1398

Abstract:

Introduction and objectives: Q fever is a zoonotic disease caused by Coxiella burnetii that considered a public health problem in different societies. Cattle, sheep and goats are the major reservoirs of this disease; infected animals excrete the bacteria in milk. An effective and rapid method for diagnosis of C. burnetii is nested-trans PCR method. The aim of this study was determination of C. burnetii in Bulk Tank Milk (BTM) samples from Kerman dairy cattle herds. Materials and methods: In this study, 48 BTM samples including 4200 industrial and semi industrial dairy cattle was collected. DNA was extracted using a commercial kit and, then, examined by nested-trans PCR method for the presence of C. burnetii. Results: Six of 48 samples (12.5%) were positive. Given the importance of the bacteria in economy of livestock and public health, its rapid and accurate diagnosis is highly important. Conclusion: Results of this study showed that the cattle milk can be a potential reservoir of C. burnetii in southeastern Iran.

Authors

Fazele Rad

Department of Pathobiology, Faculty of veterinary medicine, Shahid Bahonar University of Kerman, Kerman, Iran

Hossein Shakib

Department of Pathobiology, Faculty of veterinary medicine, Shahid Bahonar University of Kerman, Kerman, Iran

Darya Foolady

Department of Pathobiology, Faculty of veterinary medicine, Shahid Bahonar University of Kerman, Kerman, Iran

Mohammad Khalili

Department of Pathobiology, Faculty of veterinary medicine, Shahid Bahonar University of Kerman, Kerman, Iran