Yersinia ruckeri strains Population diversity in rainbow trouts from West Azarbaijan by DGGE technic

Introduction and Objectives: Yersinia ruckeri is a gram-negative stain bacillus and the causative agent of salmonids’ enteric redmouth disease. the present research aimed to study the probable strain diversity of Yersinia ruckeri by DGGE (Denaturing Gradient Gel Electrophoresis) technic and evaluate the prevalence of the disease in West Azarbaijan’ rainbow trout farms. Materials and Methods: In this regard firstly 124 individual infected fish were obtained from south of West Azarbaijan province. using biochemical tests65 samples were detected as positive samples which were subsequently tested by PCR method of 16S rRNA fragment. using the recent molecular method only 40 samples were confirmed. Results: Finally, at this study, the prevalence of Yersiniosis in West Azarbaijan’ were obtained 31.74 percent. also, the variation of the experiment, from 40 samples, 37 samples (92.5 percent) as biotype 1 and number 3 cases (7.5 percent) were identified as biotype 2. Conclusion: In a complementary study by DGGE technic showed that the variation of Yersinia ruckeri strain not any variation in the population of Yersinia ruckeri in all the samples that obtain from West Azarbaijan.

Keywords:

Yersinia ruckeri , DGGE , rainbow trout