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Overproduction of the pro-inflammatory cytokines by peripheral blood mononuclear cells from Helicobacter pylori-infected patients with peptic ulcer

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Year: 2019
COI code: MEDISM20_328
Paper Language: English

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Authors Overproduction of the pro-inflammatory cytokines by peripheral blood mononuclear cells from Helicobacter pylori-infected patients with peptic ulcer

  Maryam Nemati - Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
  Nadia Kazemipoor - Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.

Abstract:

Introduction and Objectives: H. pylori is one of the most prevalent infection in human and chronic infection with this bacterium may lead to peptic ulcer (PU), gastric mucosa-associated lymphoid tissue (MALT) lymphomas and gastric cancer. The host immunologic and genetic parameters play a critical role in the expression of the bacteria-related clinical symptoms. This investigation aimed to evaluate the expression pro-inflammatory cytokines IFN-γ and IL-12 by peripheral blood mononuclear cells (PBMCs) from H. pylori-infected peptic ulcer (PU) patients and asymptomatic (AS) subjects. Material and Methods: The fresh PBMCs were obtained from 20 PU patients, 20 AS subjects and 20 non-infected individuals and were cultured for 32 hours without stimulation, in the presence H. pylori-derived crude extract or PHA. Then levels of the IFN-γ and IL-12 in the supernatants were measured using ELISA method. Results: No significant differences were observed between HP-stimulated PBMCs and non-stimulated PBMCs from non-infected individuals concerning the IFN-γ and IL-12 production. In both PU and AS groups, the levels of IFN-γ and IL-12 production by HP-stimulated PBMCs were significantly higher than non-stimulated PBMCs. The IFN-γ and IL-12 similarly expressed in non-stimulated PBMCs and PHA-stimulated PBMCs from PU patients, AS subjects and non-infected individuals. The amounts of IFN-γ and IL-12 production by HP-stimulated PBMCs from PU were significantly higher than equal cultures in AS subjects and non-infected individuals. Conclusion: The HP-stimulated PBMCs from PU produce higher amounts of IFN-γ and IL-12 than AS subjects and non-infected individuals that represent the possible contribution of these cytokine in the pathogenesis of PU.

Keywords:

H. pylori, Peptic ulcer, IFN-γ, IL-12

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COI code: MEDISM20_328

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Nemati, Maryam & Nadia Kazemipoor, 2019, Overproduction of the pro-inflammatory cytokines by peripheral blood mononuclear cells from Helicobacter pylori-infected patients with peptic ulcer, 20th International Congress of Microbiology of Iran, كرمان, دانشگاه علوم پزشكي كرمان, https://www.civilica.com/Paper-MEDISM20-MEDISM20_328.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Nemati, Maryam & Nadia Kazemipoor, 2019)
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Type: Azad University
Paper No.: 6755
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