COMPARATIVE EFFECT OF BIOACTIVE PEPTIDES ON TESTIS HISTOLOGICAL VARIATION BELOW HEAT STRESS IN MALE RATS

Background and Aim : Scrotal hyperthermia has been known as a cause of male infertility. Testicular heat stress even for a single short period can induce destruction in testicular tissue and spermatogenesis disturbances. Antioxidants play a basic role in both food systems and the human body, reducing oxidative activities. Peptides are biological molecules with small chains of amino acids that have high anti-inflammatory and antioxidant properties. In particular, fish protein hydrolysates (FPH) with antioxidant properties have become a topic of great interest for pharmaceutical and health foods.Methods : In present study, 35 adult male Wistar rats were randomly categorized into 5 groups; group 1 served as a control, groups 2 was heat-stressed (43 ◦C for 20 min per day). group 3,4 and 5 were heat-stressed along with Bioactive Peptides (5,10 and 20 mg kg-1 day, Oral Gavage, respectively). The HS was induced through immersion of experimental rat scrotums in a water bath. After 35 days, the testicles were harvested, then dehydrated, cleaned in xylene and embedded in paraffin wax. Thin sections were cut and stained with hematoxylin and eosin for histopathological analysis in all experimental groups.Results : In HS-exposed group, histological Alterations of Seminiferous tubules showed significant decrease in a temperature-dependent manner compared to the control group. There was no change in group 3 and 4 duos to severe tissue damage, but in group 5, Bioactive Peptides have positive effects on histological factors.Conclusion : In HS-exposed group, histological Alterations of Seminiferous tubules showed significant decrease in a temperature-dependent manner compared to the control group. There was no change in group 3 and 4 duos to severe tissue damage, but in group 5, Bioactive Peptides have positive effects on histological factors.

Keywords:

Heat stress , BioactivePeptides , Seminiferous tubules , Rat