Bacteriophage Oriented Immobilization for E coli Bacteria Detection in free label Method

Immobilization of bio-molecules on substrate is the most important in bio-conjugation and medical industry. In most time, the structure of bio-molecules was changed or their functionality was loose, in immobilization step. Also, we can optimize the immobilization factors for achievement of high efficiency. In this study, we immobilized T4 bacteriophage on gold electrode by streptavidin/biotin linker for E coli bacteria detection. This immobilization has two steps: a) immobilization of streptavidin on gold electrode and b) attachment of biotin on bacteriophage surface. In first step, the streptavidin by EDAC/NHS on gold electrode then, the biotin modified bacteriophage conjugated by streptavidin. The results are studied by SEM. Then, the bacteriophage immobilized on gold electrode biosensor was patterned as a two electrodes configuration. Consequently, the E. coli trapping by oriented bacteriophage biosensor was reported by capacitance method in LCR meter.

Keywords:

E. coli bacteria , bacteriophage , capacitance , electrode