Gene Expression (mRNA) Profile of Apoptotic Related Genes in MCF-7 and T47D Breast Cancer Cells Treated by Cisplatin

Breast cancer is one of leading causes of mortality worldwide. Cisplatin is a traditionalcancer drug commonly used in chemotherapy for killing breast cancer cells. Modulation at themRNA levels of apoptotic related genes often correlate with the sensitivity of various types ofcancer cells to drugs. In this study, MCF-7 and T47D cells were treated with variousconcentrations of cisplatin for 24 h. Then, cell viability was assessed using MTT assay andIC50 was determined. RNA was extracted and cDNA was synthesized. For apoptotic andreference genes, specific primers were designed by primer express software v.3.0 target/control gene expression ratios were calculated by 2 -∆∆Ct formula. The Real-time PCRresults showed that BAX pro apoptotic gene expression was increased in MCF-7 and T47Dcells. However we found no significant change in the BAD pro apoptotic gene expression.The gene expression levels in the two cancer cells were found to be similar in relation toBAD and BAX mRNA expression whereas significant different was observed on theexpression of BCL2L12. The results showed that cisplatin exerted a dose-dependentinhibitory effect on the viability, via regulation of apoptotic gene expressions, in MCF-7 andT47D cells.

Keywords:

BCL2L12 , BAX , BAD , Real-Time PCR , Apoptotic Genes