Designing and Evaluation a New Smart Immunotoxins for Targeting Cancerous Nerve Cells

Brain tumors are a rare disease, however survival of their patients is generally poorcompared to other cancers. Moreover, irrespective of common problems and side effects incancer therapy, blood-brain barrier is a major obstacle for drug delivery in this cancer.Considering, developed new strategies to overcome this barrier in one hand, and targettherapy to cancer cells on the other hand, could be promising approaches which may beprovided via smart immunotoxins designing. Bearing in mind, designing and evaluation anew generation of smart immunotoxins with these features were considered in this study. Inthis regard, expression profiles of cell-specific antigens of brain tumor with correspondingligands were investigated. Binding affinity and structure of the ligands were characterized.Profile of the bacterial toxins with cell death capacity as well as their molecular mechanismsand structure were determined. Finally, a series of genetic constructs were assembly basedon desired domains and evaluated by a numerous of in-silico software. Our results, lead todetection a series of strains of the bacteria with low toxicity and new motifs of toxins with celldeath capacity. Moreover, this investigation introduced a precise ligand for targetingcancerous nerve cells, and a candidate domain for crossing the blood-brain barrier.Meanwhile, a series of new genetic constructs with (GGGGS)3 linker are designed, whichsome of them showed suitable quality in structure and function as new anticancer drugs,which should be further evaluation.Keywords: Brain, Tumors, Blood-Brain

Keywords:

Brain , Tumors , Blood-Brain Barrier , Target Therapy , In Silico Biology