Cytotoxicity And Apoptosis In Breast Cancer Cell Line Exposed To An Extract Of The Scrophularia Umbrosa Dumort

Publish Year: 1395
نوع سند: مقاله کنفرانسی
زبان: English
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NASTARANCANSER02_131

تاریخ نمایه سازی: 22 دی 1396

Abstract:

Breast cancer is the most common cancer in women worldwide, especially in developing countries.Scrophularia Umbrosa Dumort is an Iranian medicinal plant which is used for various disorders intraditional medicine. In this study we investigated the anti-cancer and cytotoxic effects of plantroot extracts on human breast cancer and normal cell lines. Six extracts of plant including themethanol, dichloromethan, water, butanol, ethyl acetate, petroleum ether were evaluated.Cytotoxicity effect of the extracts on MCF-7(breast cancer cell line) and NIH3T3 (normal fibroblastcell) was investigated by MTT assay. In addition apoptotic induction was measured using flowcytometry. The results showed that the water and butanol extracts had no cytotoxic effects butmethanol, dichloromethan, ethyl acetate, petroleum ether extracts significantly showed cytotoxiceffects in a dose dependent manner on the breast cancer cell line. The ethyl acetate has the lowestIC50 on cancer cell line (400μg/ml) that differs extremely from the IC50 for NIH cells (1500 μg/ml).Flow cytometric analysis showed apparent increase of apoptotic cells in mentioned extract. Ourfindings suggest that this plant may contain potential bioactive compound(s), especially ethylacetate extract, that can be used in the treatment of breast cancer.

Authors

Leila Etemad

Pharmaceutical Research Center; Mashhad University Of Medical Sciences; Mashhad, Iran

Mostafa Mansouri

Department Of Pharmacology And Toxicology, Islamic Azad University, Pharmaceutical Science Branch, Tehran, Iran