Association Of VEGF -634G> C(Rs2010963) Gene Polymorphism With Breast Cancer Risk In Northwest Of Iran

Breast cancer is one of the most prevalent cancers between women worldwide, that is considered asmain cause of cancer-related death in females. In many investigations and researches that weredone in different areas, significant association was observed between VEGF -634G> C polymorphismand breast cancer risk that is due to most important rolls of this gene in angiogenesis and finallyregenerating the new vessels in breast cancer cell nourishment. Due to specific importance of thisgene in angiogenesis, we investigated this single nucleotide polymorphism in 60 DNA specimenfrom both patients with breast cancer and normal persons by isolating DNA from peripheral bloodby salting out method, and genotypes were determined and assessed by the use of RFLP-PCR(Restriction fragment length polymorphism polymerase chain reaction) technique. The data wereanalyzed by javastat online statistics software, using Chi-square (v2) with a significance level of0.05. In this study ,CC recessive genotype frequency was higher in case group than the control group(45% and 28.33% respectively) and statistical significant difference was observed (OR=3.206, 95%CI=1.69-6.112, P=0.102). GC genotype frequency in control and case groups was 20 and 30 percentrespectively, which was significant in control group (OR=0.483, 95% CI=0.257-0.905, P=0.014). GGdominant genotype frequency was higher in control group than the case group (51.66 and 25percent respectively) (OR=0.583, 95%CI=0.289-1.173, P=0.0001). G allele frequency in case andcontrol groups was 40 and 61.66 percent respectively (OR=2.413, 95% CI=1.316-4.439, P=0.002). Callele frequency in case and control individuals was 60 and 38.33 percent respectively (OR=0.41595% CI=0.225-0.760). CC genotype in case group had significant increase, GG genotype in controlsubjects had significant increase. C allele in case individuals and G allele in control group hadsignificant increase.