Involvement Of Intrinsic Apoptotic Signaling Pathway In 4T1 Breast Cancer Cells By Sargassum Muticum PolysaccharideFraction

Publish Year: 1395
نوع سند: مقاله کنفرانسی
زبان: English
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NASTARANCANSER02_217

تاریخ نمایه سازی: 22 دی 1396

Abstract:

The Sargassum muticum is receiving great interest due to its beneficial biocompounds with highpharmacological potential. Meanwhile, rare information is reported on anti-cancer efficacy ofpolysaccharide fraction from this marine seaweed. This study was designed to evaluate the effectsof S. muticum polysaccharide fraction on 4T1 metastatic breast cancer cells. The polysaccharidefraction was extracted by simple and un-expensive method. 4T1 cells were cultured in RPMI 1640medium containing 10% FBS. The cytotoxic effects of the polysaccharide fraction of S. muticum on4T1 cancer cells was investigated using MTT assay. Pre-apoptotic efficacy was measured byfluorescence microscopy using Acridine Orange- Propodium Iodide staining. In addition,modulation of apoptotic pathway was assessed by caspase -3, -7, -8 assays. Further, wound healingassay was performed to assess anti-invasiveness effect of S. muticum polysaccharide fraction. FTIRand phenol sulfuric acid method elucidated polysaccharide extraction. It was shown that extractedpolysaccharide (15-500 μg/ml) significantly induced toxicity on 4T1 cells after 4, 48 h (as dosedependent manner). Further, AO/PI assay were recorded apoptotic DNA fragmentation subsequentto S. muticum polysaccharide fraction exposure. Caspase -3, -9 enzyme activities suggestedstimulation of intrinsic apoptosis pathway in4T1 cancer cells, suggesting pro-apoptotic potential ofmarine flora extracted polysaccharide. Wound healing assay demonstrated that blockage of 4T1 cellinvasion in a concentration dependent manner under exposure with isolated polysaccharide.Ultimately, we believe S. muticum polysaccharide fraction can serve as a useful cancer therapy forthe 43 treatment of metastatic breast cancers, but complementary investigation was required forclinical applications.

Authors

Mohammad Nabiuni

Department Of Cell And Developmental Biology, Kharazmi University, Tehran, Iran

Elaheh Amini

Department Of Animal Biology, Faculty Of Biological Sciences, Kharazmi University, Tehran, Iran

Mahnaz Fathi

Faculty Of Biological Sciences, Kharazmi University, Tehran, Iran