Antioxidant activity of silver nanoparticles synthesized via green chemistry in amaranthus cruentus seed extract.

Publish Year: 1396
نوع سند: مقاله کنفرانسی
زبان: English
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NASTARANCANSER03_124

تاریخ نمایه سازی: 7 اسفند 1396

Abstract:

In modern societies, due to exposure to contaminated environments, radiation, unhealthy foods, free radicals, various diseases affect humans, including cancer. Antioxidants are substances that protect thehuman body against free radicals and prevent cancer cells. The aim of this study is to investigate the antioxidant effect of silver nanoparticles synthesized by the green method in Amaranthus cruentus seedextract. DPPH (2,2-diphenyl-1-picrylhydrazyl) chemical is used to measure the antioxidant activity of the substances. DPPH which is solved in 96% ethanol in order to form free radicals, which its maximum absorbance at 517 nm. If silver nanoparticles which synthesized in Amaranthus cruentus seed extract has antioxidant activity, resulted in reducing free radicals and decreasing amount of. The concentrations of nanoparticles were 125, 250, 500, 1000 μg/ml. ABTS test has also used. ABTS(2,2 - azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) solved in potassium persulfate in order to form freeradicals. This solution has the peak absorbance at 734 nm. If silver nanoparticles has antioxidant properties absorbance was decreased. silver nanoparticles synthesized in Amaranthus cruentus seedextract with the help of green chemistry, was able to inhibit ABTS and DPPH free radicals, which the values of IC50 were 400 μg/ml and 500 μg/ml, respectively. findings indicated that silver nanoparticlesynthesized in Amaranthus cruentus seed extract, is able to inhibit free radicals in different concentratios, which increasing of the concentration result in more inhibitory effects. According to theresults, it is recommended to use these kind of compounds in antioxidant nutritional supplements

Authors

Mohsen Baghani

Department Of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Ali Es-Haghi

Department Of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Masoud Homayouni Tabrizi

Department Of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran