Use of bacterially derived human factor VIII (hFVIII) - light chain sub-fragment to develop specific antibody against the plasma derived hfVIII
Publish place: 04th National Biotechnology Congress of Iran
Publish Year: 1384
نوع سند: مقاله کنفرانسی
زبان: English
View: 1,768
متن کامل این Paper منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل Paper (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دریافت نمایند.
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
NBCI04_173
تاریخ نمایه سازی: 30 دی 1386
Abstract:
A 942 bp DNA coding for an epitope - containing fragment of human coagulation factor VIII (HFVIII) light chain, C1C2, was over produced in Escherichia coli under the bacteriophage T7 promoter. The expressed peptide of about 37 KD, wich cary a (Hit)6-tag on its C-terminal, appeared to be insoluble. Therefore, it was dissolved after treatment with either urea or guanidine hydrochloride and subjected for the purification based an a two-step chromatograpgy peocedure. Using immunoblotting experiment, it was documented that rabbit polyclonal antibodies, raised against the purified bacterially expressed hFVIII sub-fragment recognize the native plasma derived hFVIII. Both the recombinant hFVIII-light chain subfragment as well as its specific polyclonal antibody has provided valuable tools for studies concerning the stricture of hFVIII and its related medical applications.
Keywords:
Authors
Amir Amiri Yekta
National Institute for Genetic Engineering & Biotechnology, Tehran & Islamic Azad University of Jahrom
Alireza Zomorodipour
National Institute for Genetic Engineering & Biotechnology, Tehran
Mahvash Khodabandeh
National Institute for Genetic Engineering & Biotechnology, Tehran
Morteza Daliri Chopari
National Institute for Genetic Engineering & Biotechnology, Tehran