Bioremoval of lead by isolated Pseudomonas sp of oil contaminated soils, Khuzestan
Publish place: 8th national biotechnology congress of I.R. Iran & 4th National Conference on biosecurity
Publish Year: 1392
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
NBCI08_0677
تاریخ نمایه سازی: 29 شهریور 1394
Abstract:
In the past decade, enter the pollutants such as heavy metals have been increased in vital ecosystem. That is serious danger for life on the Earth. Heavy metals in most parts of the world are pollutants in various physicochemical forms and should purify. In this research we evaluate some methods for biological treatment for lead removal by related resistant strains.To conduct the study, five samples of oil contaminated soils of Khuzestan zones were collected under sterile conditions and transferred to laboratory immediately. The soil samples were homogenized and diluted by sterile saline till to 10-10 and cultured on Luria Bertani agar medium containing 5ppm lead nitrate. Resistant strains were isolated after 24 hours of incubation, and for isolating appropriate strains, cultured on Macconkey agar medium. Isolated bacteria were identified by biochemical tests. From total of 24 strains of isolated Pseudomonas, 10 strains were resistant to lead. Then, the MIC test was used for screening of resistant strains on Luria Bertani agar medium. Absorption tests of metal were A top strain was removed 90% of lead.
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Authors
HOMA Khorasani
Student of master. Department of biology, Islamic azad university, Tonekabon,Iran
MANSOUR Meybodi
Microbiology professor. Department of biology, Islamic azad university, Tonekabon,Iran
ZAHER Heshmatipour
Microbiology professor. Department of biology, Islamic azad university, Tonekabon,Iran
Mahbobeh Mohamadi
Student of master. Department of biology, Islamic azad university, Tonekabon,Iran
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