Analysis of the Effect of Subcutaneous Injection of Omental-Derived Cells in Healing of Third Degree Burns in Rats

Introduction: Burn injury is considered a global health issue. Third degree burn wounds do not heal spontaneously and require skin grafts. Some factors could contribute to wound healing. In this study we assessed the effect of non-fatty omental cell in burn wound healing.Material and methods: Similar third degree burn wounds were induced on the back of 48 rats, out of which 12 rates were put in a control group that did not receive any treatment. The rest of the rats were put in 3 groups, each receiving different treatment regime. Group 2 had daily application of silver sulfadiazine; Group 3 were injected with omental cells and group 4 rats were injected with phosphate buffer saline (PBS) once, followed by daily application of Vaseline to the burned region. Parameters such as open epidermis length, number of epidermal cell layers, Granulation Tissue thickness (GTT) and neutrophil density were evaluated in each groupResult: The average open epidermis length in the omental cell group was less than the other groups in day 10 and 20 (P<0.05). The thickness of epidermal cell layers in the group receiving cells was more than other groups on all days. On 20th day, there was a significant difference in GTT between the four groups (P<0.05).Conclusion: The injection of non-fatty omental cells has positive effect on third degree burn wounds in rats.