Detection of Class I, II and III integrons and analysis of Antibiotic-resistance in Staphylococcus aureus isolated from burn patients

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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NCBMED08_121

تاریخ نمایه سازی: 18 تیر 1398

Abstract:

Introduction: Staphylococcus aureus is one of the important causes of nosocomial infections and a broad variety of infections such as skin, soft tissue and many other infections. In spite of the treatment of various antibiotics, infections caused by these bacteria are still significant and the transmission of antibiotic resistance genes, particularly by Integron structures, exacerbates this issue. The object of this study was to investigate the antibiotic sensitivity pattern, the presence of class 1 and 2, and 3 integrons, and eventually to examine its potential connection with the change of resistance genes in S.aureus bacteria isolated from burn patients.Methods: Sampling for this study was carried out over a 6 months period from various clinical samples at the Amir-Al-Momenin. Hospital. 134 isolates of S.aureus were confirmed by phenotypic investigations and then tested by PCR for the presence of integrin classes 1, 2 and 3, and including the presence of eap gene for molecular recognition of isolates species.Results: The overall currency of MRSA strains in this study was 43.2%, and the prevalence of class 1, 2 and 3 integrons was 53 (39.3%) and (3.5%) 5 and 0 respectively. The identification of antibiotic resistance and the presence of class I and 2 introns for 7 and 5 antibiotics in this study was described to be significant with regard to the value of p <.05 .Conclusion: The results show the possibility of the association among antibiotic resistance and the presence of the integrons, and therefore, the appearance of integrons and given resistance cassettes through them is critical in managing the hospital infections produced by this bacterium.

Authors

Mahrokh Rajaee

Burn and Wound Healing Research Center, Shiraz University of Medical Science, Shiraz, Iran

Amir Emami

Burn and Wound Healing Research Center, Shiraz University of Medical Science, Shiraz, Iran

Neda Pirbonyeh

Burn and Wound Healing Research Center, Shiraz University of Medical Science, Shiraz, Iran

Abdollah Bazargani

Department of Bacteriology and Virology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran