Silver-nanoparticles effect as efflux pumps inhibitor on multi-drug resistance bacteria isolates from burn patients

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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NCBMED09_076

تاریخ نمایه سازی: 25 اسفند 1398

Abstract:

Background and Aim : Background: The universal problem of bacterial resistance to antibiotic reflects a serious threat for physicians to control infections. Evolution in bacteria, results in the development of various complex mechanisms such as efflux pumps, in neutralization the bactericidal effect of antibiotics. Although Metallic nanoparticles represent a potential candidate to bactericidal activity, evaluation of their mechanism will be helpful in how to apply them in clinical situations. One of the probable mechanisms of these particles is to block the bacterial efflux pumps. In this study, the effect of silver nanoparticles was evaluated on the efflux pump inhibition in clinical isolates of Acinetobacter baumannii and Pseudomonas aeruginosa. Methods : Methods: In this study, clinical strains of P. aeruginosa and A. baumannii were collected from burn patients. Antibiotic susceptibility testing of the isolates was performed after biochemical and selected of antibiotic resistance isolate. Ethidium bromide (EB)-agar cartwheel method (2 mg/l concentration) was determined for phenotypic detection of efflux pumps. This technique was repeated for all MDR studied isolates after treatment with silver nanoparticles. Results : Results: Total of 100 Gram-negative MDR isolates (Pseudomonas aeruginosa (n=50), Acinetobacter baumannii (n=50)) were studied. The efflux activity was observed in 94% and 70% of Acinetobacter and Pseudomonas isolates. Efflux pump inhibitor activity of AgNPs was observed in 12 isolates of Acinetobacter and 20 isolates of Pseudomonas with minimum inhibitory concentrations of 256 and 562μg/ml against bacterial study respectively. Conclusion : Conclusions: High-level multidrug antibiotic resistance can occur as a result of efflux pumps activity combined with other mechanisms of resistance. The application of nanoparticles as efflux pump inhibitors will help to revive the bactericidal effect of conventional antibiotics and decrease the dose of drugs and either saving high spectrum antibiotics.

Authors

Neda Pirbonyeh

Department of microbiology, Burn and Wound Healing Research Center, Microbiology Department, Shiraz University of Medical Sciences, Shiraz, Iran.

Abdolkhalegh Keshavarzi

Burn and Wound Healing Research Center,Shiraz University of Medical Sciences, Shiraz, Iran.

Sedighe Moradi

Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Amir Emami

Department of microbiology, Burn and Wound Healing Research Center, Microbiology Department, Shiraz University of Medical Sciences, Shiraz, Iran.