A novel enzymatic biosensor for the determination of Methanol

Methanol is a serious toxic component for humans which can cause permanent blindness by destruction of the optic nerve. An electrochemical enzyme-based biosensor plane has been designed by the self-assembly technique for determination of methanol in herbal distillates samples, which was composed of self-assembled thiodibutyric acid monolayer and Alcohol oxidase and peroxidase enzymes. The electrochemical methanol biosensor exhibited a linear relationship between target concentration and oxidation current in the range of 25-400 ppm and its detection limit was 10 ppm. We also measured methanol content in several commercially available herbal distillates samples with the biosensor. The accuracy of results was verified by using GC measurements, which showed good agreement with results from proposed biosensor.

Keywords:

Methanol , Biosensor , Oxidase , Peroxidase , Herbal Distillate