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Application and set up PCR-RFLP method for investigation of IL-28B rs8099917 G/T allele distribution

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Year: 2012
COI code: NCNCMB01_168
Paper Language: English

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Authors Application and set up PCR-RFLP method for investigation of IL-28B rs8099917 G/T allele distribution

  Maryam Karkhane - Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical sciences, Tehran, Iran
  Pedram Azimzadeh - Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical sciences, Tehran, Iran
  Seyed Reza Mohebbi - Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical sciences, Tehran, Iran
  Samaneh Ahmadi - Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical sciences, Tehran, Iran

Abstract:

Background and aim: Single nucleotide polymorphisms rs8099917 located in 8kb upstream of IL-28B gene. Also it have been confirmed that genetic polymorphisms in the IL-28B gene region are strongly associated with the response to treatment and open the possibility of a personalized medicine for the treatment of the progressive disease. In this study, we investigate set up amplification of rs8099917 fragments .with specific designed primers by RFLP-PCR method Methods: PCR-RFLP technique was used to genotyping of controls. Sequences of SNP rs8099917 were found in Pubmed website and endonuclease restriction enzymes was prepared by NEBcutter tools web- .site. Primers were designed by Gene Runner software Results: In this study, we set up amplify sequences of SNPs fragments by PCR-RFLP for n=79 cases. Fragments of PCR and RFLP were sharp and could be detected genotype of samples (GG/GT/TT) .Conclusion: The PCR-RFLP is simple and useful method for investigation of genotyping of SNPs of IL-28 gene for study samples and can help to treatment. Therefore this method can be enhanced the prediction treatment of some virological patients which can help to clearance

Keywords:

IL-28B gene, rs8099917, PCR-RFLP

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COI code: NCNCMB01_168

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Karkhane, Maryam; Pedram Azimzadeh; Seyed Reza Mohebbi & Samaneh Ahmadi, 2012, Application and set up PCR-RFLP method for investigation of IL-28B rs8099917 G/T allele distribution, 1st National Conference on New Cellular Molecular Biotechnology, پرند, دانشگاه آزاد اسلامي واحد پرند, https://www.civilica.com/Paper-NCNCMB01-NCNCMB01_168.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Karkhane, Maryam; Pedram Azimzadeh; Seyed Reza Mohebbi & Samaneh Ahmadi, 2012)
Second and more: (Karkhane; Azimzadeh; Mohebbi & Ahmadi, 2012)
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Paper No.: 16371
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