COI code: NCNTA02_007
Paper Language: English
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Authors Effect of Silver Nanoparticles On Grapevine Leaf Explants Sterilization at In vitro ConditionsAli Gouran - Ms Student, Dept. of Horticultural Sciences, University of Kurdistan
Mohammad Jirani - Ms Student, Dept. of Horticultural Sciences, University of Kurdistan
Ali Akbar Mozafari - Ms Student, Dept. of Horticultural Sciences, University of Kurdistan
Mahmud Koshesh Saba - Ms Student, Dept. of Horticultural Sciences, University of Kurdistan
Abstract:The success in all of plant tissue culture protocols is related to decontamination of explants. The purpose of the current study was to evaluate new compounds for the control of external and internal contamination and their impact on different grape cultivars in process of tissue culture. In this study leaf explants of grape Vitis vinifera L. (cv. Rashe , Khoshnave and Farkhi ) was used. Silver nanoparticles used for disinfection of explants surface in the process of tissue culture. In this studyethanol 95 % for 30 seconds + silver nanoparticles (100, 500 and 1000 ppm) used for surface disinfection during 10 and 20 minutes. The results showed low rates of burned explants in nanosilver treatments. Generally the explants tissues had high level of tolerance to silver nanoparticles. Silver nanoparticles treatments had moderate and high effect on bacterial contamination control, but had moderate effect for fungi contamination control. The best result was obtained with increasing concentrations of silver nanoparticles and time of disinfection.
Keywords:Grape, Silver Nanoparticles, Sterilization, In vitro, Leaf explants
COI code: NCNTA02_007
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Gouran, Ali; Mohammad Jirani; Ali Akbar Mozafari & Mahmud Koshesh Saba, 2013, Effect of Silver Nanoparticles On Grapevine Leaf Explants Sterilization at In vitro Conditions, 2nd National Conference on Nanotechnology from theory to application, اصفهان, موسسه آموزش عالي جامي, https://www.civilica.com/Paper-NCNTA02-NCNTA02_007.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Gouran, Ali; Mohammad Jirani; Ali Akbar Mozafari & Mahmud Koshesh Saba, 2013)
Second and more: (Gouran; Jirani; Mozafari & Koshesh Saba, 2013)
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Type: state university
Paper No.: 4340
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