Production of Nanocellulose from Acetobacter and Gluconobacter isolated from vingers and juices

Background and objectives: the microbial cellulose which is produced by some strains of Acetobacter and Gluconobacter is structurally similar to plant cellulose .based on this feature, the microbial cellulose has been used in medical and industrial productions as an important raw material. The aims of this study were to isolate the native bacteria species producing cellulose and Nanocellulose evaluate their production rate from local vingers and fruit juices.Material and methods: in this study, 24 samples were collected from different types of local vinegars, juices were cultivated in Hestrin-schramm s agar medium. Afterward the isolates with maximum ability to produce cellulose were selected and identified using phenotyping tests, biochemical tests, PCR method and Sequensing method. After purification of the cellulose, eynzymatic digestions method by cellulose enzyme and Atomic Force Microscope were used to confirm the presence of cellulose.Results: totally, 6 strains with the cellulose production ability were isolated, of them 4 strains with maximum production ability were selected for further investigation. Base on biochemical tests and sequencing method. These isolates were classified as Acetobacter xylinum, Acromobacter spanius and Pseudomonas durifulva. In addition, it was proved the determined Nanocellulose fiber contains a diameter of about 600-1000 nm.Conclusion: according to the findings, the produced Nanocellulose by these species has suitable traits in comparison with standard strains. The cellulose can be produced by native isolates other than Acetobacter and Gluconobacter.

Keywords:

Nanocellulose , cellulose , Acetobacter , Gluconobacter , vinger