The Effects of Electromagnetic wave in Radiofrequency Range on the Temperature Responses of Glutamate Oxidase Based Biosensor

Introduction: Glutamate is a powerful excitatory neurotransmitter that is released by nerve cells in the brain. For this reason, detection of glutamate is of importance in both clinical samples. In this research for measurement of glutamate release a glutamate biosensor is fabricated using immobilizing of glutamate oxidase at the surface of platinum electrode. Since the electromagnetic wave may be affect the immobilized enzyme function, this fact is studied by determination of optimum Temperature of fabricated biosensor performance. Material and methods: For investigation of 915 MHz electromagnetic wave effect on glutamate oxidase function, the enzyme was irradiated in GTEM cell wave guide for 60 minutes. Then irradiated enzyme and nano chitosan immobilized at the surface of platinum electrode. For determination of optimum temperature, the responses of fabricated biosensor at various temperature was evaluated between 25±1 °C and 75±1 °C at pH 7.4 in buffer phosphate 0.1 M in presence of 0.5 mM glutamate using cyclicvoltametry method with 10 mv/s scan rate. The responses of fabricated biosensor with non-irradiated and irradiated enzymes were compared.Results and conclusion: The results showed that in both fabricated biosensors using non-irradiated enzyme and irradiated enzyme, the responses gradually increased 25±1°C up to 45±1 ºC, afterwards, the biosensor has lost its activity. This phenomenon might be due to thermal inactivation of glutamate oxidase. Also, the results showed that the best response was happened at 25°C, so that, this temperature was chosen as working temperature for all further experiments. It is important to note that, these results alone cannot confirm the effect of 915 MHz waves on the structure and function of the glutamate oxidase enzyme

Keywords:

Radiofrequency , Glutamate oxidase , Exposure