Correlation of miR-622 downregulation with development of multiple sclerosis

Introduction: miRNAs play an important role in certain types of biological processes by regulating geneexpression. Deregulated expression of different miRNAs is associated with the onset and progression of certaindiseases such as immune and neurological disorders. Evidence underlines the importance of miRNAs in thepathogenesis of multiple sclerosis (MS). In this study the expression profile has been evaluated by GEOmicroarray data, GSM587543, which consist of miRNAs profile of peripheral blood from MS patients.Therefore, this study aimed to compare the miR-622 expression level in MS patients with health control.Methods: In this case-control study, 60 individuals (30 cases with MS and 30 healthy controls) were enrolled.Followed verifying disease 2 ml peripheral blood was given from all subjects. Total RNA was extracted andcDNA was synthesized by stem-loop method. The relative gene expression was determined using quantitativereal-time RT PCR (qRT-PCR).Results: The expression of mature miR-622 was lower in MS patient compared to healthy controls and it wasstatistically significant (P < 0.05). No significant association was noticed between MS type and the miR-622expression.Conclusion: Our study shown that the expression of miR-622 is lower in multiple sclerosis disorder. Accordingto this study, it can be proposed that down-regulation of miR-622 increased MS development risk and likely canbe as disease marker and a novel therapeutic target in MS. Therefore, supplementary studies should be done toelucidate the exact mechanism of miRNA action.

Keywords:

Multiple sclerosis , miR-622 , Gene expression , Biomarker