CHANGE IN MICE SEXUAL HORMONES BY FLUNIXIN INJECTION

Considering the sensitively of reproduction, mentioned factors have caused different injuries on male and female sexual systems such as infertility and low fertility which are various degree ofsexual system failures. Infertility has affected 15% of world couples which 40-50% of infertilities are because of male factors. Flunixin is a potent non steroidal, anti-inflammatory agent that is used as an analgesic and antipyretic drug in veterinary. Considering the frequent use of this drug inrecent years this study was conducted to investigate the effects of this drug on pituitary–gonadal axis of mice. Results of this study can be used highly for better use of this drug and saving sexual power of male sex. MATERIALS AND METHODSFive experimental groups with eight male mice in each group were studied as control, Placebo, andthree treatment groups. Flunixin was injected in 0.5, 1, and 1.5 mg/kg in peritoneum in a twentydayperiod. Control group didn’t receive any injection and Placebo group received only physiological serum. Completely Randomized Factorial Designs was applied to test the significance of treatments and means were compared using Duncan's multiple range test at P = 0.05. Effects offlunixin meglumine on FSH, LH and testosterone concentration were analyzed using the PROCMIXED procedure (SAS Inst. Inc., Cary, NC) with a repeated measures model that included the effects of flunixin meglumine. RESULTS AND DISCUSSIONAccording to results, FSH and LH concentrations were increased significantly (p<0.05) but testosterone was not changed. Results show that flunixin can affect sexual power of male sex byincreasing hormones of hypophysis-testicle axis. In conclusion, the best treatment to enhance FSHand LH concentration of mice, was the exact 1.5 mg/kg flunixin. This treatment can be proposed as additional substance for FSH and LH discharge increasment. Nonsteroidal anti-inflammatory drugs, such as flunixin meglumine (FM), inhibit PGF2α synthesis by inhibiting the COX-2 enzyme, andhave positive effects on pregnancy rates when administered between Days 14 and 17 post-AI (Merrill et al., 2007; Guzeloglu et al., 2007).

Keywords:

flunixin , testosterone , FSH , LH , mice