COI code: NIMED03_126
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors Enhanced Neural Differentioation of Epidermal Neural Crest Stem Cell by Synergistic Effect of Lithium Carbonate and Crocin on BDNF and GDNF Expression as Neurotrophic FactorsShirin Ahmadi - Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Mohammad Nabiuni - Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Mohammad Tahmaseb - Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Elaheh Amini - Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Abstract:Neurodegenerative diseases are incurable and debilitating conditions that result in progressive degeneration of nerve cells. Due to the complexity of conditions in neurodegenerative diseases, combination therapy including cell therapy and drug therapy is important as a new therapeutic strategy. Epidermal neural crest stem cells (EPI-NCSCs) are as one of the best option in cell therapy for various neurologicaldiseases. In this study, the effect of Lithium carbonate and Crocin, with considering of their effects on cellular signaling pathways and neuroprotective properties were investigated on expression of neurotrophic factors BDNF and GDNF in EPI-NCSCs. Materials and Methods:EPI-NCSCs were isolated from the Whisker hair follicles of the 2-week-old male Wistar rat and cultured in collagen-coated culture plates (1mg/ml). After EPINCSCs emigration, adhering cells removed by trypsin and subsequently subcultured. Then, after 72 hours oftreatment with different concentration of drugs [Lithium (0.1, 0.5, 1, 1.2, 1.4, 1.6, 1.8, 2, 4, 8 mM), Crocin (12.5, 50, 100, 200, 500, 1000, 1500, 2000 ,2500 μM) and lithium (1mM) + Crocin (12.5, 50, 100, 500, 1000, 1500, 2000 ,2500 μM)], and trial doses were selected by MTTassay. The cells were treated with selected concentration (Lithium 1 mM, Crocin 1.5 mM, Lithium 1mM and Crocin 1 mM) for 7 days. Results: The Real Time PCR results indicated an increasing in expression of BDNF and GDNF in treated cells as compared with control(*p<0.05, **p<0.01 and ***p<0.001). Conclusion: The results in this study confirmed and supported the neuroprotective/neurogenesis effects of Lithium and Crocin, it also showed that the proposed protocol can be used to increase EPI-NCSCs differentiation potential into neural cells in cell therapy and combination therapy of neurodegenerative diseases.
COI code: NIMED03_126
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Ahmadi, Shirin; Mohammad Nabiuni; Mohammad Tahmaseb & Elaheh Amini, 2019, Enhanced Neural Differentioation of Epidermal Neural Crest Stem Cell by Synergistic Effect of Lithium Carbonate and Crocin on BDNF and GDNF Expression as Neurotrophic Factors, Third International Nervous System Inflammatory Conference and Third Student Neuroscience Festival, مشهد, دانشگاه علوم پزشكي مشهد, https://www.civilica.com/Paper-NIMED03-NIMED03_126.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Ahmadi, Shirin; Mohammad Nabiuni; Mohammad Tahmaseb & Elaheh Amini, 2019)
Second and more: (Ahmadi; Nabiuni; Tahmaseb & Amini, 2019)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
The University/Research Center Information:
Type: state university
Paper No.: 9273
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- Tumefactive brain MRI lesions in NMOSD patient: report of a case
- New therapeutic approach by G2013 in experimental model of multiple sclerosis
- HEADACHE in pregnancy:diagnosis and management
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.