Potential of Tumor Suppressor miR-145 to Reduce the Expression of KLF4 in Order to Control the Progression of MCF7 BreastCancer Cell Line

Background and Aim: Recent studies indicate that miR-145 plays animportant role as a tumor suppressor and metastasis in a variety of tumor.This gene can also induce the differentiation of embryonic stem cells.KLF4 is a necessary transcription factor for maintaining the pluripotencyof embryonic stem cells. KLF4 is considered as an oncogene. Reductionof the KLF4 self-renewal property cause suppresses the cell migration andinvasion of breast cancer cells. The aim of this study is the investigationof the effect of miR-145 overexpression on KLF4 gene expression in mcf7cell line.Methods: The gene construct of miR-145 expression vector was designed;then, it was transduced to mcf7 cell line. Total RNA extraction and cDNAsynthesized. Gene expression of KLF4 measured by real-time PCR andflow cytometry technique and the result were analyzed.Results: Preliminary results indicate the KLF4 downregulation followingthe overexpression of miR-145 in mcf7 cancer cells, and inducesdifferentiation resulting in cancer cells reduction.Conclusion: The results of this study for the first-time show, the effectof miR-145 on the expression of KLF4 in a breast cancer cell line. Thisstudy, according to the theory of cancer stem cells may be effective inthe control of the progression of cancer through molecular intervention.

Keywords:

Breast cancer , KLF4 , miR-145