Analysis of the Blood Differential Test for the Prognosis of Cell-Based Therapy in Acute lung Inflammatory Phenotypes

Background and Aim: Acute respiratory distress syndrome is aheterogeneous syndrome that produces an inflammatory response, suchas elevated WBC and this can lead to acute organ dysfunction. Until nowany drug has proved beneficial in the prevention of ARDS. Mesenchymestem cells are the best-described cells and the most used as a cell therapy.Of the various cell-based therapy options, BM-MSCs having the most efficacy for lung injury. Analysis of blood samples is typically one ofthe first steps in diagnosing and is an important part of the research toprovide useful information to in diagnosis and management.Methods: This study was excluded from ten healthy Shall Sheep intwo groups of control and treatment. Bone marrow samples werecollected from the treated group, and MSCs were isolated and cultured.Then an experimental model of ARDS was induced by endotrachealadministration of E.coli-lipopolysaccharides strains O55: B5 andinflammation confirmed. After 24 hours of ARDS, 50×106 BM-MSCswere autografted in the treatment group, and PBS was injected in thecontrol group intrapulmonary. Venous blood samples were collectedin EDTA anticoagulated tubes before and after therapeutic induction.Hematological parameters were assessed with an automated analyzerinclude the hematocrit, erythrocytes, hemoglobin, platelets, whiteblood cells (WBC), heterophile, eosinophils, monocytes, basophils,and lymphocytes immediately. The CBC results were available from theanimal model at the time of the 3, 6, 12, 24, 48 and 168th hours aftercell-based therapy. The results are expressed as mean±SD for continuousvariables. Data analysis was performed using SPSS software.Results: Factors affecting levels during treatment were a determined, andinflammatory response such as elevated white-cell count, a segmentedneutrophil count showed a significant increase in the two groups afterinducing an experimental model of ARDS. In the treatment group,reduction of WBC and segmented neutrophil count were significant attimes 24, 48, 72 and 168 hours in compared with baseline and also,in compared with the control group at times 24, 48, 72 and 168 hours.Reduction of band cells was significant at 24, 48, 168 hours comparedwith the control group. Also, the Lymphocyte counts at 24 hours andmonocyte counts at 72 and 168 hours were significant. Statistical analysisin the hematocrit, platelets, eosinophil, basophile showed no importantdifference between the two groups at different times.Conclusion: Analysis of clinical blood samples is typically one of the firststeps in diagnosing disease. It has the potential when interpreted carefullyand about the clinical history, to provide very useful information to assistin diagnosis and management. In this research abnormalities in thecomplete blood count were confirmed in the inflammation time so thatthe microscopic review of blood smears from sheep to detect band cellsor segmented neutrophil change is valuable and could help in assessingdisease severity and prognosis in cell therapy approach.

Keywords:

BM-MSCs , ARDS , Blood differential test