Antioxidant Activity of Chrysin-Loaded Electrospun Nanofibrous Mats Increases Stemness Preservation of Human Adipose-Derived Stem Cells
Publish place: Third National Festival and International Congress of Rehabilitation Stem Cells and Medical Stems and Technologies
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
NSCMRMED03_306
تاریخ نمایه سازی: 30 دی 1397
Abstract:
Background and Aim: An ideal biomaterial in regenerative medicineshould be able to regulate stem cell proliferation without the loss of itspluripotency. Chrysin (Chr) is a naturally occurring flavone with a widespectrum of biological functions including anti-inflammatory and antioxidantproperties. The present study describes the influence of Chrloadednanofibrous mats on the regulation of proliferation and stemnesspreservation of adipose-derived stem cells (ADSCs).Methods: Chr-loaded PCL/PEG nanofibrous mats were produced viaelectrospinning process and the successful fabrication of these bioactivemats was confirmed by FE-SEM and FTIR. ADSCs were seeded on thenanofibers and their morphology, proliferation, and stemness expressionwas analyzed using FE-SEM, MTT, and qPCR assays after 2 weeks ofincubation, respectively.Results: The results display that ADSCs exhibit better adhesion andsignificantly increased proliferation on the Chr-loaded PCL/PEGnanofibrous mats in relative to the PCL/PEG nanofibers and TCPs. Thegreater proliferation ability of ADSCs on Chr based nanofibers was further confirmed by higher expression levels of stemness markers Sox-2,Nanog, Oct-4, and Rex-1.Conclusion: These findings demonstrate that Chr-loaded PCL/PEGelectrospun nanofibrous mats can be applied to improve cell adhesionand proliferation while concurrently preserving the stemness of ADSCs,thus representing a hopeful potential for application in stem cell therapystrategies.
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Authors
Younes Pilehvar-Soltanahmadi
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Yaghoub Deldar
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Nosratollah Zarghami
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran