Intramural Transplantation of Homologous Fibroblasts Promoted the Wound Healing in Rat Model of Intestinal Anastomosis

Background and Aim: This study was aimed to evaluate the intramuraltransplantation of homologous fibroblast on the healing of colonicanastomosis in rats by means of histopathological parameters.Methods: Thirty-six male mature Wistar rats were divided randomly intothree groups and undergone the surgical procedure of colonic resectionand anastomosis. Primary fibroblasts were isolated from the skin ofrats. The abdominal cavity of rats in the sham group was approachedby midline incision, the whole intestine was manipulated and then theabdominal wall was sutured. In both groups of control and treatment,ascending colon was exposed and a 0.5 cm section of colon was resectedand thereafter anastomosed by simple interrupted sutures. Rats in thecontrol group were received 0.5 mL PBS in the seromuscular layer andin the treatment group 0.5 mL PBS containing about 1×106 fibroblastswere injected in the same manner. On day 7, all rats were euthanizedfor sampling.Results: Histopathologic findings indicated that angiogenesis and numberof mature fibroblasts, collagen deposition, re-epithelialization, thethickness of granulation tissue and wound contraction were significantlyincreased in the treatment group and all indices were normal in the shamgroup.Conclusion: Results of the present study suggested that intramuralinjection of fibroblasts in the anastomosis site, could improve thehistopathological indices of wound healing.

Keywords:

Colon , Anastomosis , Fibroblast , Healing , Histopathology