Supplementation of sperm freezing medium with Myoinositol improve human sperm parameters and protects it against DNA fragmentation and apoptosis

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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RMED08_087

تاریخ نمایه سازی: 21 مرداد 1398

Abstract:

Background: Cryopreservation has been extensively used in assisted reproductive technology and it is an important task to improve current methods of sperm cryopreservation. Myoinositol is involved in several systemic processes and in mechanisms of signal transduction in the plasma membrane as precursor of second messengers. On the male reproductive function, MYO appears to regulate seminal plasma osmolarity and also sperm maturation, motility, capacitation and acrosome reaction. Recently an antioxidant action has also been suggested.Objective: The aim of this study is to evaluate the beneficial effect of MYO supplement in freezing media on the post thaw sperm quality.Materials and Methods: Semen samples from 40 normozoospermic men were divided into two aliquots and frozen with 2mg/ml MYO free /or supplemented freezing medium. Post thaw process, computer-aided semen analysis was used to analyze sperm motility and morphology. Reactive oxygen species (fluorometry of DCFH-DA), total antioxidant capacity, lipid peroxidation (colorimetric assay by ELISA reader) and DNA fragmentation (TUNEL staining) were evaluated.Results: MYO significantly improved progressive motility and normal morphology in treated samples (p<0.05). Lipid peroxidation can be precluded in samples frozen with MYO supplemented freezing media (p<0.05). While MYO did not affect significantly the amount of ROS (p> 0.05), it was associated with a significant increase in total antioxidant capacity (p<0.05). In MYO treated samples, DNA fragmentation was lower than control ones (p<0.001).Conclusion: The findings support the use of 2mg/ml myoinositol supplemented freezing media in sperm cryopreservation to increase sperm quality after freezing-thawing procedures.

Authors

F Mohammadi

Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran

N Varanloo

Department of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran

M Heydari Nasrabadi

Department of Biology Sciences, Parand Branch, Islamic Azad University, Tehran, Iran

A Vatannejad

Department of Cellular and Molecular Nutrition, Tehran University of Medical Sciences, Tehran, Iran