Effect of estradiol on oocyte qualitative and quantitative parameters and embryo formation following IVF in mice

Background: oocyte maturation is one of the important role in vitro fertilization. aging affects on the genome activation which is related with oocyte maturation. advanced age cause changes in oocyte inclusive cytoplasmic/ extracytoplasmic deficiencies. Studies showed use of exogenous estrogen and antioxidants can regulate apoptosis process and reduce oocyte cytoplasm abnormalies.Objective: Therefore, our aim in this study was improve the oocyte quality parameters by using appropriate doses of estradiol and sesame oil.Materials and Methods: Six groups of mice were selected, 3 groups young and 3 groups old. the control group received normal saline and exprimental group received sesame oil (10 IU) and estradiol (E2) 1 μl + sesame oil (10 IU) Respectively., all treatments was done intraperitoneally in diestrus phase. after IVF program the status of Refractile body, smooth endoplasmic reticulum, vacuole, oocyte cytoplasm polar body, previtelline space, zona pellucida thichness, oocyte number and two cells embryo were evaluated.Results: in young group, oocyte number was higher than old ones (p=0.015) But the results are beneficial with the group who received sesame oil (29.70±2.43 vs 23.10±2.43 p=0.01). The pervitiline space in the young group that received (E2) + sesame oiLwas less than the old one (p=0.015). The number of two-cell embryos in sesame oiL group was higher than other groups (p=0.009). there was no significant difference in the appearance of cytoplasm,pollar body, SER, Vacuole and Perivitelline space except the number of refract body in old group (p=0.016).Conclusion: This study demonstrate that the sesame oil as a strong antioxidant will have beneficial effects on the number of oocytes and two cell embryo in young group. the result shows that advance age dose no change in oocyte quantitative parameters except refractile body.

Keywords:

Estradiol , Sesame oil , Oocyte Morphology , Embryo formation , IVF , mice