Evaluation of the association between promoter polymorphism of Fas gene with POF

Background: Premature ovarian failure (POF) is the term usually used to describe women aged younger than 40 years, who identify with hypergonadotropic hypogonadism, amenorrhoea, and infertility. The clinical presentation is diverse, and several disorders can lead to POF. Alteration of the FAS pathway regulating cell death may lead to POF, but the effects of functional promoter polymorphisms of the FAS gene on risk of POF are unknown.Objective: The aim of present study is the evaluation of FAS polymorphisms on the risk of POF patients.Materials and Methods: We genotyped the FAS _670 A> G polymorphism in 51 case patients with POF and 60 control subjects by using PCR-RFLP method.Results: Regarding the FAS _670 the frequency of AG genotype was 47.1% and 46.7% in POF patients and control group, respectively. The AG genotype was more prevalent in the cases (47.1%) than the controls (46.7%). Also the most POF patients had AG genotype when compared to FAS _670 AA and GG genotypes. However the difference was not statistically significant (OR= 1.00, p=0.99).Conclusion: Our finding showed that the incidence of different genotype and allele frequencies of FAS _670 A/G was not statistically significant among POF patients and healthy women.

Keywords:

Premature ovarian failure , FAS , Polymorphism